chapter 9 cellular respiration mastering biology

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In glycolysis, as in all the stages of cellular respiration, the transfer of electrons from electron donors to electron acceptors plays a critical role in the overall conversion of the energy in foods to energy in ATP. These reactions involving electron transfers are known as oxidation-reduction, or redox, reactions. Drag the words on the left to the appropriate blanks on the right to complete the sentences.

1) Oxidized 2) Reduced 3) Glucose 4) Pyruvate 5) NAD⁺ 6) NADH

Suppose that a cell's demand for ATP suddenly exceeds its supply of ATP from cellular respiration. Which statement correctly describes how this increased demand would lead to an increased rate of ATP production?

ATP levels would fall at first, decreasing the inhibition of PFK and increasing the rate of ATP production.

How would anaerobic conditions (when no O2 is present) affect the rate of electron transport and ATP production during oxidative phosphorylation? (Note that you should not consider the effect on ATP synthesis in glycolysis or the citric acid cycle.) -Neither electron transport nor ATP synthesis would be affected. -Electron transport would stop but ATP synthesis would be unaffected. -Both electron transport and ATP synthesis would stop. -Electron transport would be unaffected but ATP synthesis would stop.

Both electron transport and ATP synthesis would stop. (Oxygen plays an essential role in cellular respiration because it is the final electron acceptor for the entire process. Without O2, mitochondria are unable to oxidize the NADH and FADH2 produced in the first three steps of cellular respiration, and thus cannot make any ATP via oxidative phosphorylation. In addition, without O2 the mitochondria cannot oxidize the NADH and FADH2 back to NAD+ and FAD, which are needed as inputs to the first three stages of cellular respiration.)

TP synthesis in glycolysis: substrate-level phosphorylation The ATP that is generated in glycolysis is produced by substrate-level phosphorylation, a very different mechanism than the one used to produce ATP during oxidative phosphorylation. Phosphorylation reactions involve the addition of a phosphate group to another molecule. Sort the statements into the appropriate bin depending on whether or not they correctly describe some aspect of substrate-level phosphorylation in glycolysis.

Correct: -One of the substrates is a molecule derived from the breakdown of glucose -An enzyme is required in order for the reaction to occur -A bond must be broken between an organic molecule and phosphate before ATP can form. Incorrect: -The phosphate group added to ADP to make ATP comes from free inorganic phosphate ions. -The enyzmes involved in ATP synthesis must be attached to a membrane to produce ATP.

During strenuous exercise, anaerobic conditions can result if the cardiovascular system cannot supply oxygen fast enough to meet the demands of muscle cells. Assume that a muscle cell's demand for ATP under anaerobic conditions remains the same as it was under aerobic conditions. What would happen to the cell's rate of glucose utilization?

Glucose utilization would increase a lot.

Under anaerobic conditions (a lack of oxygen), the conversion of pyruvate to acetyl CoA stops. Which of these statements is the correct explanation for this observation?

In the absence of oxygen, electron transport stops. NADH is no longer converted to NAD+, which is needed for the first three stages of cellular respiration.

From the following compounds involved in cellular respiration, choose those that are the net inputs and net outputs of glycolysis. Drag each compound to the appropriate bin. If the compound is not involved in glycolysis, drag it to the "not input or output" bin.

Net Input: ADP, NAD⁺, Glucose Net Output: ATP, NADH and Pyruvate, not input or output: O₂, CO₂, coenzyme A and acetyl CoA

In the citric acid cycle (also known as the Krebs cycle), acetyl CoA is completely oxidized. From the following compounds involved in cellular respiration, choose those that are the net inputs and net outputs of the citric acid cycle. Drag each compound to the appropriate bin. If a compound is not involved in the citric acid cycle, drag it to the "not input or output" bin. (Note that not all of the inputs and outputs of the citric acid cycle are included.)

Net Input: Acetyl CoA, NAD⁺, ADP Net Output: Coenzyme A, CO₂, NADH, ATP Not Input or Output: Pyruvate, Glucose, O₂

In the last stage of cellular respiration, oxidative phosphorylation, all of the reduced electron carriers produced in the previous stages are oxidized by oxygen via the electron transport chain. The energy from this oxidation is stored in a form that is used by most other energy-requiring reactions in cells. From the following compounds involved in cellular respiration, choose those that are the net inputs and net outputs of oxidative phosphorylation. Drag each compound to the appropriate bin. If a compound is not involved in oxidative phosphorylation, drag it to the "not input or output" bin. (Note that not all of the inputs and outputs of oxidative phosphorylation are listed.)

Net Input: NADH, ADP, O₂ Net Output: NAD⁺, ATP, CO₂ and Water Not Input or Output: Pyruvate, Glucose, Acetyl CoA, Coenzyme A and CO₂.

In acetyl CoA formation, the carbon-containing compound from glycolysis is oxidized to produce acetyl CoA. From the following compounds involved in cellular respiration, choose those that are the net inputs and net outputs of acetyl CoA formation. Drag each compound to the appropriate bin. If a compound is not involved in acetyl CoA formation, drag it to the "not input or output" bin. (Note that not all of the inputs and outputs of acetyl CoA formation are included.

Net Input: NAD⁺, coenzyme A, pyruvate Net Output: NADH, acetyl CoA, CO₂ not input or output: O₂, ADP, glucose and ATP

Energy from glycolysis Among the products of glycolysis, which compounds contain energy that can be used by other biological reactions?

Pyruvate, NADH, ATP ATP

Sort the labels into the correct bin according to the effect that gramicidin would have on each process.

Remains the same: proton pumping rate, electron transport rate, rate of oxygen uptake Decreases or goes to zero: Rate of ATP synthesis, size of the proton gradient

Carbon atoms in acetyl CoA formation and the citric acid cycle During acetyl CoA formation and the citric acid cycle, all of the carbon atoms that enter cellular respiration in the glucose molecule are released in the form of CO2. Use this diagram to track the carbon-containing compounds that play a role in these two stages.

Start: acetyl CoA - 2 C; continue clockwise: 6 C, 6 C, 5 C, 4 C, 4 C, 4 C, 4 C, 4 C

Part complete The four stages of cellular respiration do not function independently. Instead, they are coupled together because one or more outputs from one stage functions as an input to another stage. The coupling works in both directions, as indicated by the arrows in the diagram below. In this activity, you will identify the compounds that couple the stages of cellular respiration. Drag the labels on the left onto the diagram to identify the compounds that couple each stage. Labels may be used once, more than once, or not at all.

a. pyruvate b. NADH c. NAD⁺ d. NADH e. NAD⁺

Why is the citric acid cycle a cyclic pathway rather than a linear pathway? a. More ATP is produced per CO2 released in cyclic processes than in linear processes. b. It is easier to remove electrons and produce CO2 from compounds with three or more carbon atoms than from a two-carbon compound such as acetyl CoA. c. Redox reactions that simultaneously produce CO2 and NADH occur only in cyclic processes. d. Cyclic processes, such as the citric acid cycle, require a different mechanism of ATP synthesis than linear processes, such as glycolysis.

b. It is easier to remove electrons and produce CO2 from compounds with three or more carbon atoms than from a two-carbon compound such as acetyl CoA. Although it is possible to oxidize the two-carbon acetyl group of acetyl CoA to two molecules of CO2, it is much more difficult than adding the acetyl group to a four-carbon acid to form a six-carbon acid (citrate). Citrate can then be oxidized sequentially to release two molecules of CO2.

Which statement best explains why more ATP is made per molecule of NADH than per molecule of FADH2?

fewer protons are pumped across the inner mitochondrial membrane when FADH2 is the electron donor than when NADH is the electron donor.

Net redox reaction in acetyl CoA formation and the citric acid cycle. In the sequential reactions of acetyl CoA formation and the citric acid cycle, pyruvate (the output from glycolysis) is completely oxidized, and the electrons produced from this oxidation are passed on to two types of electron acceptors.

pyruvate is oxidized to (a) CO2 NAD+ is reduced to (b) NADH (c) FAD is reduced to (d)FADH2

In mitochondrial electron transport, what is the direct role of O2?

to function as the final electron acceptor in the electron transport chain


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