WBC Structure and Function

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a. Bone marrow b. Peripheral blood c. Tissues

Leukocytes are found in 3 different compartments in the body namely:

acidophil

Another name for an eosinophil

a. Phagocytes b. Immunocytes

Based on FUNCTION, leukocytes are divided in 2:

Yes, however, their granules are not their primary identifying characteristic.

Do non-granulocytes contain granules? What does the answer to this question imply about the classification of leukocytes according to granularity?

10 - 15

The diameter of a band is _____ micrometers

10-14

The diameter of a basophil is _____ micrometers

12-22

The diameter of a monocyte is _____ micrometers

10 - 15

The diameter of a segmented neutrophil is _____ micrometers

cytoplasm

The granulocytes contain distinct granules in their ___________.

Monocyte

The largest leukocyte in peripheral blood

Heterochromatin

Their amount increases with maturity; appears as coarse granular areas forming medium-sized to large clumps that are irregularly distributed.

Basophil

Their chromatin patterns are smudged and distinct.

Eosinophil

Their cytoplasm is filled with large, spherical, refractive granules of uniform size that stain bright orange-pink

Band

Their nucleus is elongated, curved or sausage-shaped with rounded ends and area of clumping at each pole; filaments not present

neutrophils

Their nucleus is segmented into 2-5 lobes connected by a thread-like filament

Eosinophil

Their nucleus is usually band-shaped or segmented into only 2 lobes

Leukocytes

They are colourless, nucleated cells that circulate in peripheral blood.

Leukocytes

They are the body's main line of defence against foreign invaders such as bacteria, viruses and other foreign antigens.

Parachromatin

They are the non-staining or clear areas between the chromatin clumps; becomes prominent as the cells mature

Polymorphonuclear cells

They contain a multi lobed or segmented nucleus in their mature form. Examples are neutrophils, basophils and eosinophils.

Mononuclear cells

They contain a nucleus that may be variable in shape but is a single mass, not segmented.

Euchromatin

This denotes immaturity and is a characteristic of blast cells; it appears finely granular and is distributed uniformly with very few if any tiny aggregates.

nuclear shape

This is a relatively poor criterion for cell identification , as it is highly susceptible to artefacts and can change considerably in disease states.

color

This is the least reliable criterion for cell identification because it varies with each staining system.

a. Monocytes b. Lymphocytes

What are the 2 leukocytes which are non-granulocytes?

a. Neutrophils b. Eosinophils c. Basophils

What are the 3 leukocytes which are granulocytes?

a. Neutrophils b. Eosinophils c. Basophils d. Monocytes

What are the 4 leukocytes which are phagocytes?

a. Neutrophils b. Eosinophils c. Basophils d. Monocytes e. Lymphocytes

What are the 5 principal types of leukocytes that normally circulate in peripheral blood?

light pink

What colour does a neutrophil's cytoplasm stain

dark purple

What is the colour of an Eosinophil's nucleus?

Stained peripheral blood film using a Romanowsky-type stain

What is the major routine method for studying the morphology of leukocytes?

Chromatin pattern

What is the most valuable and reliable criterion for deciding whether a cell is mature or immature

1-5%

What is the normal value of bands?

<1%

What is the normal value of basophils?

1-5%

What is the normal value of eosinophils?

50-70%

What is the normal value of segmented neutrophils?

The nuclei of immature polymorphonuclear cells are not segmented and because basophil nuclei do no necessarily segment.

Why isn't the classification of Leukocytes according to nuclear segmentation satisfactory enough?


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