Bio 101- Labs 1-9 Chapter 1

Jackie went to the sink at the end of lab to wash her hands. After removing her watch and rings, she turned on the water, wet her hands, added soap and thoroughly rubbed and lathered her hands for 30 seconds, cleaning the backs of her hands, her fingernails, and between her fingers. She then turned off the water, and reached for the paper towels to dry her hands. Identify the correct statement below.

Jackie should have used paper towels to turn off the faucet.

Jacob had just finished his lab work and went to the sink to wash his hands. He removed his watch, and added liquid soap to his hands. He then turned on the water and began to rub his hands together, lathering for 20 seconds and cleaning fronts and backs of hands, fingernails, and between his fingers. He then rinsed his hands in running water, dried his hands with paper towels, and used an additional paper towel to turn off the faucet. Which statement is correct?

Jacob should have wet his hands in running water before applying the soap

Identify the correct statements about lab coats.

Lab coats are worn in lab so that students can be correctly identified as biology students, Lab coats provide some protection against contamination with infectious microorganisms, Lab coats provide some protection against exposure to corrosive chemicals and lab coats are taken home for decontamination

Which of the statements below describe appropriate use of paper towels when washing hands?

Paper towels are used to apply soap and work up a good lather, Paper towels are used to dry hands after washing, Paper towels are used to turn ON the faucet, Paper towels are used to turn OFF the faucet and Paper towels are used to thoroughly clean the finger nails,

The best source of information regarding the hazards posed by any chemical and specific handling instructions is the

Safety Data Sheet (SDS)

Who and or what is protected by lab safety protocols ?

Students doing lab activities, lab instructors and technical assistants, anyone who walks into the labs and the physical labs space and the equipment housed within the lab

When should soap be added to the hands?

after the hands are wet

Laboratory instruments are sterilized prior to reuse by __________.

autoclaving (steam autoclave)

When should hands be washed?

before starting lab activities, after completion of lab activities, after removing gloves worn during labs activities and before using the microscope

Which is the most appropriate disposal for a swab used to culture your throat during lab?

biohazard bag

sharps container

broken glass

Goggles or appropriate safety glasses protect the eyes from

chemical splashes, fumes of preservatives used on anatomy specimens and UV exposure when using UV radiation in the laboratory

Which piece of equipment is routinely used for the decontamination of infectious laboratory waste?

steam autoclave

long hair should always be tied back when in the laboratory

true

biohazard bag

used culture plates

Which of these steps will prevent the immediate recontamination of the hands after washing?

using a paper towel to turn off the faucet handle

Identify which activity should be avoided prior to starting an experiment in the microbiology laboratory.

wearing open toed shoes

How many biosafety levels are there for selected infectious agents?

four

slides rack for autoclaving

glass culture tubes containing broth cultures of Staphylococcus epidermidis

Which of the following items should be autoclaved?

glass culture tubes, hazardous chemicals, noncontaminated paper waste and chemical waste from doing stains

Which of the following are potential hazards which you might encounter in the laboratory?

infectious microorganisms, corrosive chemicals, sharp tools or broken glass and gamma radiation

normal trash receptacle

paper towels used to dry hand after hand washing

Which items can be discarded in a regular trash can?

paper towels used to wipe up bacterial spills, uncontaminated waste paper and used glass slides

Lab coats, gloves, face masks, and goggles are all examples of

personal protective equipment

Hand washing once just prior to labs is the only time hand washing is recommended to avoid personal contamination.

False

How long should the hands be lathered with soap and water?

20 seconds

Which of the following represent important safety procedures for microbiology students?

Always wear open-toed shoes so organisms do not become trapped in shoes. , Wash your hands before and after laboratory procedures. and Disinfect your lab bench at the beginning and end of the lab period.

What biosafety level is designated for organisms that don't usually cause disease in humans?

BSL-1

Which of the following items can be washed in the sink after use in the laboratory?

dissection tools, glassware that was not used with hazardous chemicals, E. coli broth culture tubes, inoculating loops and dissection trays

Which of the following items may be on the laboratory bench top while doing lab work?

equipment items specifically needed to complete the lab activity , backpack/purse, pencil/pen and cell phone

If gloves are worn in lab, hand washing is unneceesary.

false

Most chemicals used in the laboratory can be discarded in the sink after use.

false

Only laboratories BSL-3 and higher require fire extinguishers emergency showers and eye wash station

false

Thoroughly washing hands will sterilize them.

false

Using a cell phone in lab is permissible since it can be used for timing experiments.

false