Microscope

What is the low magnification?

4x

What are the four magnification levels on a microscope?

4x 10x 40x 100x

0.5 mm = _____ μm

500 μm

250 μm = _____ mm

0.25 mm

1000 μm= ______ mm

1 mm

10000 μm = _____ mm

10 mm

1 mm= _____ μm

1000 μm

What is the oil immersion magnification?

100x

What is the middle magnification?

10x

3 mm= _____ μm

3000 μm

What is the high dry magnification?

40x

If the diameter of field of view of a light microscope at 40x magnification is 6000 micrometer (microns), what would be the field of view at 400x magnification? (m1d1=m2d2)

40x6000μm=400x x=40.6000μm/400 x=600μm

Why are microscopes important for studying biological specimens?

Biological molecules are made of cells. Cells are microscopic to the eye. Microscopes allow us to see things much closer.

What changes occur when you go from lower to higher magnification?

Increase in size Light intensity decreases Field of View decreases Depth of field decreases Resolution increases

What is the advantage of observing a specimen at total magnification of 400x?

The image is clearer

What is the disadvantage of observing a specimen at total magnification of 40x?

The magnification and resolutions are smaller

What is the advantage of observing a specimen at total magnification of 40x?

You can see more of your specimen

What is the disadvantage of observing a specimen at total magnification of 400x?

You see less of the specimen

What does oil immersion mean?

the specimen must have a layer of specific oil on top when under the 100x magnification