microscopy post-lab assignment

How many micrometers (μm) are in 12.8 nanometers (nm)? **DO NOT ROUND YOUR ANSWER**

0.0128

How many centimeters (cm) are in 134.8 micrometers (μm)? **DO NOT ROUND YOUR ANSWER**

0.01348 cm

If the diameter of your microscope field is 1 mm at low power (10×) and your ocular lens is 10×, then what is the diameter of your field when you switch to the 50× objective lens? 2 mm 0.2 mm 0.02 mm 0.002 mm

0.2 mm

What is the TOTAL MAGNIFICATION of a microscope with the following setup? Ocular lens magnification = 4X Objective lens magnification = 48X

192X

If a circular object seen in your low-power field (diameter 1 mm) occupies about 1/4 of the diameter of the field, the object's diameter is about ________. 250 μm 25 μm 2.5 μm 0.25 μm

250 μm

What is the TOTAL MAGNIFICATION of a microscope with the following setup? Ocular lens magnification = 5X Objective lens magnification = 60X

300X

If the ocular lens magnifies a specimen 10x, and the objective lens used magnifies the specimen 35x, what is the total magnification being used to observe the specimen? __________

350X

How many millimeters (mm) are in 35.9 cenitmeters (cm)?

359 mm

If your ocular lens is 10×, and you are using your 45× objective lens to view a slide, the total magnification is ________.

450X

How many nanometers (nm) are in 57.9 millimeters (mm)?

57900000

What is the TOTAL MAGNIFICATION of a microscope with the following setup? Ocular lens magnification = 4X Objective lens magnification = 20X

80X

Considering the information below, which objective lens has the better resolution? Objective Lens A: Magnification = 100X Objective Lens B: Magnification = 50X

Objective Lens A

Considering the information below, which objective lens has the largest field of view? Objective Lens A: Depth of field = 0.24 mm Objective Lens B: Depth of field = 1.2 mm

Objective Lens B

Considering the information below, which objective lens has the shortest working distance? Objective Lens A: Field of view = 6.2 mm Objective Lens B: Field of view = 1.8 mm

Objective Lens B

A blood smear is used to diagnose malaria. In patients with malaria, the protozoa can be found near and inside red blood cells. Explain why a microscope capable of high magnification and high resolution would be needed to diagnose malaria.

Structures inside a cell including protozoa are very small and cannot be seen with low magnification.

Histopathology is the use of microscopes to view tissues to diagnose and track the progression of diseases. Why are thin slices of tissue ideal for this procedure? Thin slices are easier to prepare. Thin slices are used because thicker tissue slices are more difficult to stain, and it is harder to see cellular details. Thin slices are used so that pathologists can clearly see individual cells for their assessment. Too many layers of cells makes it difficult to focus and hard to see what changes have occurred in the tissues. Thick sections would waste a lot of material and would require larger patient samples.

Thin slices are used so that pathologists can clearly see individual cells for their assessment. Too many layers of cells makes it difficult to focus and hard to see what changes have occurred in the tissues.

The microscope field is the ________.

area you see through the lenses of the microscope

You are looking at very lightly stained cells under the microscope, and you want to increase the contrast so that you can see them "better." You ________ the iris diaphragm to ________ the light. close, dim open, dim close, increase open, increase

close, dim

The ________ is a substage lens that concentrates light on the specimen. objective lens condenser iris diaphragm lever light source

condenser

As total magnification increases, the depth of field ________.

decreases

As you increase the total magnification of the specimen, the size of the field ________. remains the same increases decreases

decreases

As you increase the total magnification of the specimen, the working distance ________.

decreases

The thickness of the sample that remains in focus at each magnification is called the... depth of field. working distance. resolution .magnification. parfocal. field diameter.

depth of field

This is the formula for calculating the diameter of an unknown microscope field. diameter of field A + total magnification of field A = diameter of field B + total magnification of field B diameter of field A + diameter of field B = total magnification of field A + total magnification of field B diameter of field A × diameter of field B = total magnification of field A × total magnification of field B diameter of field A × total magnification of field A = diameter of field B × total magnification of field B

diameter of field A × total magnification of field A = diameter of field B × total magnification of field B

True or False: You should always begin observation of specimens with the oil immersion lens

false

The amount of the area of the sample that can be seen in the ocular lens is called the... parfocal. magnification. working distance. field diameter. depth of field. resolution.

field diameter

When using the higher power objective lenses, you would use this part of the microscope to focus the specimen. course adjustment knob fine adjustment knob iris diaphragm lever

fine adjustment knob

When observing a slide under a microscope, begin focusing with the ________-power objective lens in place. highest midrange lowest

lowest

You begin your observations using the ________ lens and the ________ adjustment knob.

lowest-power, coarse

The working distance for a 10× objective lens is ________.

much greater than the working distance for the 100× objective lens

The total magnification of an object can be found by ________.

multiplying the power of the objective lens by the power of the ocular lens

The ________ of the microscope carries three or four objective lenses. nosepiece ocular arm base

nosepiece

The ability of a microscope to stay in focus when switching between objectives is called... magnification. parfocal. field diameter. depth of field. resolution. working distance.

parfocal

You have learned that the objective lens magnifies the object on the slide, producing the ________ image, and that the ocular lens magnifies that image, forming the ________ image. objective, real real, virtual ocular, objective virtual, ocular

real, virtual

The ability to distinguish between two points as being separate is called... working distance. magnification. field diameter. depth of field. resolution. parofcal

resolution

The ability to discriminate two close objects as separate is called ________. working distance resolution (or resolving power) parfocal virtual image

resolution (or resolving power)

Before putting away the microscope in the storage cabinet you must observe all of the following except ________. wrap the cord around the microscope clean the lenses rotate the highest power objective lens in position remove the slide from the stage

rotate the highest power objective lens in position

Microscope specimens have depth, as well as length and width. If you are focused on the middle layer of cells and wish to see the top layer of cells clearly, you should move the ________. slide to the left or right slide further away from the lens slide toward or away from you slide closer to the lens

slide further away from the lens

The microscope slide rests on the __________ while being viewed. iris base stage condenser

stage

When you want to study a slide under the microscope, you place it on the ________.

stage

To transport a microscope, hold it ________.

upright, with one hand on its arm and the other hand supporting its base

How do you clean the lenses of your microscope?

with special lens paper and cleaner

The distance between the bottom of the objective lens and the sample when the sample is in focus is called the... field diameter. resolution. working distance. depth of field. magnification. parfocal.

working distance

You are looking at a slide of three crossed threads. Yellow is on the bottom, blue is in the middle, and red is on the top. When you rotate the adjustment knob forward (away from you) on your microscope, the stage rises. You move the adjustment knob to focus on the middle thread. As you rotate the adjustment knob forward, which color thread will come into focus next? red yellow blue

yellow

You are observing the letter "e" under a compound microscope. Which of the following indicates how the image appears as you view it through the ocular lens? e Ε ɘ ə

ə