6.4 Vocab/Review
A section of the template strand of DNA reads: 5'-CAACACTAA- 3'. Determine the mRNA sequence and translate it.
3'-GTTGTATT-5'
Referring to question 10, imagine if the coding strand was transcribed and translated instead. Determine the mRNA sequence and translate it. How would this error affect the polypeptide produced
3'-GUUGUGAUU-5', there would be completely different amino acids
The very first tRNA will enter at which site i'm the ribosome?
E
How does transcription and translation differ between prokaryotes and eukaryotes
Eukaryotic Transcription takes place inside of the Nucleus, while Prokaryotic Transcription takes place in the Cytoplasm. In Eukaryotic Transcription, proteins known as Transcriptional factors are needed, while In Prokaryotic Transcription, no such proteins are needed.
If the tRNA anticodon reads 3'- CUU -5 ' then what does the mRNA codon read that it can bind to ? Knowing this , what amino acid will the tRNA be carrying
GAA; Glu
An mRNA sequence reads 5'- CGAUGCAGA 3 ' . Translate this sequence (Assume you are downstream of the start codon )
GCUACGUCU
Which amino acid does tRNA initially carry?
Met (the start codon; AUG)
Trace the path of a newly produced polypeptide that will leave the cell
Once a protein is made at a ribsome its going to enter the Rough ER and be modified, from there it goes to the Golgi to be packaged and modified, then sent somewhere else in or out of the cell.
Translation
Process by which mRNA is decoded and a protein is produced
What is the codon that tRNA initially binds to?
UAC
tRNA has a ____________ region which is complementary and antiparallel to ___________
anticodon; codon
Stop codon
codon that signals to ribosomes to stop translation (UAA, UAG, UGA)
Summarize the purpose of translation
controls cell behavior and gene regulation because proteins are the functional molecules that determine cell types and function
Anticodon
group of three bases on a tRNA molecule that are complementary to an mRNA codon
Where does translation occur
ribosomes
Protein synthesis occurs in two stages. __________ and _________
transcription and translation
tRNA
transfer RNA; type of RNA that carries amino acids to the ribosome
When does translation stop
when a stop codon is reached