Biomedical Science Exam pt.2

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Which of the following is NOT found in DNA?

chromosome

What type of chemical bond is found between paired bases of the DNA double helix

hydrogen

Cytosine makes up 38% of the nucleotides in a sample of DNA from an organism. Approximately what percentage of the nucleotides in this sample will be guanine?

38%

What evidence found at a crime scene would contain DNA?

Blood

Given the DNA strand GAATTCCTCGAG, what would be the complimentary strand to make the double stranded DNA?

CTTAAGGAGCTC

which of the following bases is a purine?

Guanine

DNA is a very large molecule (a ploymer) made up of a long chain of repeating sub-units called ____________.

Nucleotides

In DNA profiling by gel electrophoresis, DNA is separated on the basis of?

Number of base pairs (BP)

Scientists are able to produce millions of copies of a specific DNA sequence from a small amount of DNA through the process of:

Polymerase Chain Reaction (PCR)

What characteristic separates DNA fragments during gel electrophoresis?

The length of the DNA fragment

What property of DNA causes it to migrate to the positive pole of the electrophoresis apparatus?

The negative charge of the DNA

The smaller the DNA fragment, the:

further it moves down the gel

When running a gel electrophoresis, DNA will migrate towards the ________ end because DNA has a __________________ charge.

positive, negative

DNA is cut at specific nucleotide sequences by

restriction enzymes

gel electrophoresis is used to separate the fragments of DNA. The larger pieces of DNA will travel __________ the smaller fragments through the gel.

slower than


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