Blood Bank 2 (Test 1) - HDN/Kleihauer Betke
Kleihauer-Betke Test
a quantitative test that approximates the volume of fetal cells in mother's circulation.
Kleihauer-Betke Test
Fetal Hgb is Hgb F, which is NOT acid soluble
Rosette Test
Fetal bleed screen test (FBST)- qualitative testing with test principle: D Pos Fetal cells + REagent Anti-D + D Pos indicator=Rosettes cells.
Rh HDFN
NO spherocytes and positive DAT, Intrauterine and Exchange Transfusion are which HDFN?
Kleihauer-Betke Test
Normal adults have under .4% Hgb F.
Hemolytic Disease of the Fetus and Newborn
ABO and Rh HDFN are types of?
Kleihauer-Betke Test
Adult Hgb is A or A2, which ARE acid soluble.
Rh HDFN
Caused by maternal Anti-D. Requires treatment to correct anemia and high bilirubin.
Hemolytic Disease of the Fetus and Newborn
Clinical Findings: - Enlarged liver and spleen. - Cardiac failure, secondary to anemia. - Edema "hydrops fetalis" - Jaundice after birth - Brain damage may result
ABO
Most common form of HDFN and is usually subclinical
ABO
Most common in Group O mothers with Group A or B infant.
ABO
Most frequently involved antibody is IgG Anti-AB. Spherocytes seen.
Kleihauer-Betke Test
This test is important for determining need for multiple doses of RhIG, and it can also be used as a screening test. The test is also called Acid Elution Stain method.