Blood Bank 2 (Test 1) - HDN/Kleihauer Betke

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Kleihauer-Betke Test

a quantitative test that approximates the volume of fetal cells in mother's circulation.

Kleihauer-Betke Test

Fetal Hgb is Hgb F, which is NOT acid soluble

Rosette Test

Fetal bleed screen test (FBST)- qualitative testing with test principle: D Pos Fetal cells + REagent Anti-D + D Pos indicator=Rosettes cells.

Rh HDFN

NO spherocytes and positive DAT, Intrauterine and Exchange Transfusion are which HDFN?

Kleihauer-Betke Test

Normal adults have under .4% Hgb F.

Hemolytic Disease of the Fetus and Newborn

ABO and Rh HDFN are types of?

Kleihauer-Betke Test

Adult Hgb is A or A2, which ARE acid soluble.

Rh HDFN

Caused by maternal Anti-D. Requires treatment to correct anemia and high bilirubin.

Hemolytic Disease of the Fetus and Newborn

Clinical Findings: - Enlarged liver and spleen. - Cardiac failure, secondary to anemia. - Edema "hydrops fetalis" - Jaundice after birth - Brain damage may result

ABO

Most common form of HDFN and is usually subclinical

ABO

Most common in Group O mothers with Group A or B infant.

ABO

Most frequently involved antibody is IgG Anti-AB. Spherocytes seen.

Kleihauer-Betke Test

This test is important for determining need for multiple doses of RhIG, and it can also be used as a screening test. The test is also called Acid Elution Stain method.


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