Cell Bio Final Exam New material

Place the following events of M phase into the correct order. 1. Alignment of the chromosomes at the spindle equator 2. Attachment of microtubules to chromosomes 3. Breakdown of nuclear envelope 4. Reformation of the nuclear envelope 5. Condensation of the chromosomes 6. Separation of sister chromatids

5,3,2,1,6,4

Which of the following statements is CORRECT?

A gain-of-function mutation in only one allele of oncogene can stimulate cell survival or division.

Which of the following statements is FALSE?

A loss-of-function mutation of telomerase will convert a normal cell to a cancer cell.

Which of the following statements about cancer is FALSE?

A mutation in a single cancer-critical gene is sufficient to convert a normal cell into a cancer cell.

Which of the following statements about apoptosis is TRUE?

Apoptosis can be promoted by the release of cytochrome c into the cytosol from mitochondria.

an animal cell that has 8 chromosomes in G1 phase. How many of each of the following structures will the cell have during mitosis? sister chromatids / centromeres / kinetochores / centrosomes

B. 16/16/16/2

In an experiment, you treated cultured cells with colchicine, then analyzed these cells using flowcytometry. Which peak in the result would be dominant?

B. II

Suppress of apoptosis can promote cancer cell survival. Which of the following compound could be an anti-cancer drug?

C. A compound that inhibits Bcl2

The following genes could become oncogene except .

C. BAD

These phase contrast images shows a living cell at differentstages in M phase. Oder them into the correct sequence.

C. E-D-C-A-F-B

Which of the following compounds can be a potentiality-cancer drug? A compound that inhibit

C. E2F & E. S-Cdk

Identify the phases of cell populations in I/II/III.

C. G1/G2/S

A hematopoietic stem cell in the bone marrow is _________.

C. Multipotent

What would be the consequence of inactivation of E2F?

Cells would be unable to enter the S phase.

Which number in the figure is labeled correctly?(P: phosphorylation of kinase activity site)

D. 7 - M-Cdk

In DNA replication the lagging strand is synthesized discontinuously at the replication fork because

DNA polymerase can polymerize nucleotides only in the 5'-to-3' direction.

If the anaphase promoting complex (APC/C) is temperature sensitive (active at 30 degree, but inactive at 40 degree), shifting to the higher temperature (40 degree) at the start of M phase would prevent which of the following?

Degradation of securin and separation of sister chromatids.

Irradiated mammalian cells usually stop dividing and arrest at a G1 checkpoint. Place the following events in the order in which they occur. I. production of p21 II. DNA damage III. inhibition of cyclin-Cdk complexes IV. activation of p53

II-IV-I-III

Which of the following statements is TRUE?

In anaphase B, microtubules associated with the cell membrane shorten.

A particular strain of yeast produces strains with a mutant cdc2 (M-Cdk) gene, resulting in little to no production of the M-Cdk protein kinase. As a result, what would be expected?

None of these.

Which of the following transcription factors is needed to convert an adult cell into an induced pluripotent stem (iPS) cell?

Oct4 Klf4 Sox2

In the cell containing a loss-of-function mutation of the separase gene, which of the following phenotypes can be predicted?

Sister chromatids will not be segregated after metaphase.

Which of the following muta1ons may suppress cell prolifera1on?

a mutation in the NLS (nuclear localization signal sequence) of β-catenin

A multipotent cell _________.

can give rise to some cell types in the body

An adult hematopoietic stem cell found in the bone marrow ______________________.

can undergo self-renewing divisions for the lifetime

A cell with mutated lamins that cannot be phosphorylated in M phase will be unable to

disassemble its nuclear lamina at prometaphase.

A malignant tumor is more dangerous than a benign tumor because its cells ________________.

invade other tissues

Embryonic stem cells are such a powerful possible treatment for disease because they are______.

pluripotent

When a terminally differentiated cell in an adult body dies, it can typically be replaced in the body by a stock of ________.

proliferating precursor cells that are derived from adult stem cells

Progression through the cell cycle requires a cyclin to bind to a Cdk because _________.

the binding of a cyclin to Cdk is required for Cdk enzymatic activity