Exam 1
You wish to study a previously undiscovered organism. Which data would give you a quick overview of what the organism transcribes to compare with other, known organisms?
cDNA sequencing
Discovered that DNA has equal amounts of Adenine and Thymine
chargaff
incorporation of amino acids into a growing polypeptide has stopped, which one of the following molecules is most likely lacking in the cell?
charged tRNA
Which of the following represent basic research?
describing the structure of kinetochores, identifying the molecules involved in translation and transcription
In the scientific method, once you have proposed a testable hypothesis, what is the next step?
design a controlled experiment
which of the following represents peer review?
discussing the outcome of an experiment with another student, noticing that the lab protocol has an error, reading a manuscript for a class discussion
Which of the following is more closely related to mRNA?
expressed sequence tag
Griffith discovered that the transforming material was RNA? T/F
false
RNA polymerase II is required for tRNA synthesis T/F
false
RNA polymerase III is required for mRNA synthesis T/F
false
Watson and Crick discovered that DNA was replicated in a conservative manner? T/F
false
prokaryote DNA is linear in form T/F
false
prokaryote RNA has multiple origins of replication T/F
false
discovered the helical form of DNA
franklin
Which of the following is needed to perform PCR?
free nucleotides, DNA polymerase
Which of the following molecules is not needed for DNA replication using PCR?
helicase
In the Avery et al. experiment, what would it have meant if the removal of polysaccharides from the heat-killed IIIS S. pneumoniae cells had led to the living mouse?
it would have shown that the transforming material for mice was polysaccharides
Which of the following carry out their functions in the cytoplasm?
mRNA
The process associated with DNA polymerase is:
mitosis
Which of these Hardy-Weinberg assumptions has changed the most in Humans over the past 300 years?
natural selection
which of the following are required for a population to be in genetic equilibrium?
no mutation, no natural selection, sufficient population size (aka no genetic drift), random mating, no migration
You notice a missing chromosome in a patients karyotype - which of these conditions could be caused by this monosomy?
none of the above
after reading a manuscript, you realize that they used the wrong Human population in their study. This is an example of
poor performance of the research
Molecules required to make a polypeptide
rRNA, mRNA, tRNA, helicase
You have performed a test on a patient and found that the mRNA of a suspect gene in the pathway has a normal transcription level; Which of the following could be the cause of the disease?
siRNA is reducing suspect gene polypeptide production, microRNA is reducing suspect gene polypeptide production, the suspect gene mRNA encodes a defective polypeptide
The temperature required to separate two complementary strands of the same DNA molecule is affected by which of the following factors?
the G/C content of the strand
you have performed an extraction of a eukaryote cells' RNA - you discover that there are no mRNA molecules - what is the most likely cause of this?
the RNA polymerase II genes have been inactivated
What would cause all transcription to stop in a prokaryote
the sole RNA polymerase gene has mutated
Commonly used name for a PCR machine
thermal cycler
The process associated with RNA polymerase
transcription
Process interrupted by siRNA
translation
The process associated with ribosomes is:
translation
this process regulated by microRNA is
translation
a mutation is important for which of the following fields of genetics?
transmission genetics, population genetics, the central dogma
A heated lid on a PCR machine is used to keep your samples from evaporating T/F
true
Avery, Macleod and McCarthy discovered that DNA was responsible for Griffiths' transformation experiment? T/F
true
Eukaryote DNA has multiple origins of replication (True/False)
true
housekeeping genes are always expressed T/F
true
In a diploid cell, mitosis leads to:
two daughter cells
discovered that DNA was the genetic material
Avery, MaCleod, McCarthy
RNA polymerase is not required to make which of the following
DNA
Molecule required for meiosis I and mitosis
DNA polymerase, DNA Template, Helicase, Free Nucleotides
Which process does require complementary base pairing?
DNA replication, siRNA replication, transcription, translation
Which of the following represent applied research?
Finding a cure for Alzheimer's, Describing the structure of kinetochores, identifying the molecules involved in translation and transcription
You need this molecule to make microRNA
RNA polymerase
You have detected a 10-fold decrease in siRNA production in human cancer cells vs a control.
a specific cellular control gene is no longer being silenced
You are studying a new organism. You notice that there are more unique polypeptides than genes - what could be the reason for this?
alternative splicing
Your genome of interest has a 40% G/C content. What is the percent of the genome that is Guanine?
20%
The following is an mRNA sequence. What was the CODING sequence? 5'- AUCCAAAACGUUGA - 3'
5'- ATCCAAAACGTTGA - 3'
Given the following DNA coding sequence 5' - CGATGATGCCTCATTGT - 3', what is the resulting polypeptide in single letter annotation (beginning from the start codon only)?
5'-MMPHC...-3'
Which of the following represents the best possible (not likely to be random)"e" value from a BLAST result?
7e-150
Discovered the structure of DNA
Watson and Crick