Lab #1 Isolation of Bacteria from the environment
-What temps are Petri dishes incubated inverted? Why is this? -Why are plates inverted? WHat happens in they are not inverted?
- 37'C in order to speed up the growth of microbes deposites on the surface of that agar - Plates are inverted because moisture will be forming during the incubation that will settle on the lid. If not inverted the moisture will fall back on the agar and compromise the results.
-Define Colony Morphology *Look and Try to identify and name the different parts of the chart -In many cases is colony description enough to identify the name of the organisms?
- Colony Morphology: the description of size, shape,color, texture, etc. of each individual colony that grow on the plate -Yes
*Methods* -What is the Petri dish filled with? -What happens to the medium? And under what conditions does it happen in? -What do you do the inoculate the dish? -What do you do with the swab? -After all the procedures , how do label the plate?
- Filled with media -The medium is allowed to gel under sterile conditions and as long as the Petri dish remains closed, it should have remained sterile - To inoculate the dish, the lid is removed and is exposed to the air for about 20 minutes - A moistened swab is used to swab a surface and then run over the agar surface to deposit the microbes onto the medium - Label the bottom of the Petri dish, close to the edge of the bottom in order to make it easier to read the label and to be able to observe what is happening in the plate
*Results* -How long does it take results to show? -What will you examine on the Petri dish? -What do you look for when when analyzing the results of the Petri dish ? (2)
-24-48 hours -Will be examined for different types of microbial growth -Analyzing the growth on the plate includes a description of the colony morphology and count of eah type of colony
-Where is Broth media often put? -Where is Agar media often put? -In all cases what happens to the media components?
-Broth media put in a sealed tube (broth tube) -Agar media put into tubes( slants) or Petri dishes (plates) -Media Components are dissolved in water and sterilized
-Define Medium (p1: Media) >What does it provide? >What form does it come in? > How are liquid media made into solid form?What does it come to look like? > What is liquid media called? What is solid media called?
-Medium (p1: Media): material that is used to grow microorganisms in a laboratory >Provides all nutrients necessary for the proper groeth of a particular microbe > Solid or liquid form > Liquid media are made into a solid form by adding agar. Forms a gelatin-like substance > Broth: Liquid media / Agar: solid media
-Are microbes found in air and surfaces? -What does the growth of bacterial dependent on? -What do TSA agar in Petri dishes do for the growth of microbes?
-Yes -Growth of bacteria would be dependent on the composition of the media we used -encourages the growth of microbes
Define Sterile
Free from living organisms
What are the most widespread forms of life >Where can they live?
Microbes > Live where larger organisms cannot survive
What consequences might arise from the presence of these microbes in the environment?
One consequence that can arrive from the presence of these microbes is that it could cause other living things such as plants to rot, or die. Another consequence can be that it can cause human or animals to get sick if they come into contact with these microbes.
What is the role of digests in the medium? the agar?
The role of the digests in the medium is to provide nutrients for the proper growth of microbes. The role of digests in agar is to make the liquid medium into a solid form in order to help the microbes grow. Agar also allows us to be able to view the growth of the microbes.
Define the term colony as it refers to microbial growth.
The term colony refers to each group of fungi and bacteria that have grown from a single mother cell, and formed on the plate. (clone of bacteria all genetically alike)
