Micro Lab MidTerm - Microscopy
Which part of the microscope will be used first to adjust the focus when starting with the lowest-power lens?
Coarse focus
Staining cells such that their color is different from the background enhances which quality of the cells?
Contrast
What is enhanced when a stain is used to make a sample, or parts of a sample, darker than the background in a slide?
Contrast
Which of the following is defined as the ability to distinguish an object from the background?
Contrast
Resolution is an image quality factor that involves being able to see fine ________, which is essential for image clarity.
Details
Which of these challenges are microscopes used to overcome?
Many biological specimens are too small to see with the unaided eye.
Which metric unit is most appropriate for expressing the size of human skin cells?
Micrometer
The total magnification of an image is determined by ______ the magnification power of the ocular lens and the magnification power of the objective lens used.
Multiplying
The lens on the microscope that is primarily responsible for the magnification of the specimen is the ____ lens.
Objective
The goal of microscopy is to create a magnified image of
Objects that are too small to be seen with the unaided eye.
Which part of the bright-field microscope is a lens that is located closest to the eye during use?
The ocular lenses
What is the name for the part of a bright-field microscope that displays the specimen?
The stage
What is the goal of microscopy?
To create a magnified image of objects that are too small to be seen with the unaided eye
The portion of a slide that is visible through the eyepiece is called the field of
View
The portion of a slide that is visible through the eyepiece is called the field of:
View
Resolution is an image quality factor that involves being able to see fine _______ , which is essential for image clarity.
Wet mount
Which microscopy method is beneficial because of its applications for living specimens?
Wet mount
The function of immersion oil in microscopy is to
direct more light rays to the objective lens.
Bright-field microscopes use a combination of lenses and ________ to view a specimen.
lights
Select the components used by a bright-field microscope to view a specimen.
1. A light source 2. Glass lenses
Select the specimen that could be viewed using a light microscope.
1. Cells 2. Tissues 3. Bacteria
Which metric units are most appropriate for expressing the size of macroscopic organisms?
1. Meters 2. Centimeters
Select the benefits of wet mount sample preparation for microscopy.
1. Motility of specimens can be observed. 2. The preparation is relatively simple and fast. 3. Living organisms can be viewed.
4x 10x 40x 100x
1. Scanning objective 2. Low power objective 3. High power objective 4. Oil immersion
Select all of the following that can be studied with bright-field microscopy.
1.Bacteria 2. Tissues 3. Cells
If a specimen is viewed using a 100× objective and the ocular lenses, what is the total magnification?
1000x
Oil immersion microscopy uses which objective lens?
100x lense
Which objective lens is used with a sample that has been prepared with a wet mount?
10× low-power lens
A benefit of using the oil immersion lens is a reduction in scatter, which
Field of view
The portion of a slide that is visible through the eyepiece is called the
Field of view
Bright-field microscopes use a combination of
Glass lenses and light to view a specimen.
Which lens has a magnification of 40×?
High-power objective
Changing the magnification of an image with a microscope changes the portion of the slide that is visible, or the
Increase resolution
Which phenomenon is reduced by oil immersion microscopy?
Loss of light that scatters into the air or onto the slide
Which lens has a magnification of 10×?
Low power objective
If you are unable to see something with your eyes, you can increase the _____ to make it look bigger.
Magnification
Which lens has a magnification of 100×?
Oil immersion
Increasing the _____ would allow you to tell if what appears as one object is really two objects extremely near each other.
Resolution
Which image quality factor is defined as the ability to distinguish fine detail?
Resolution
Which part of the bright-field microscope focuses light on the specimen?
The condenser
