Micro Lab MidTerm - Microscopy

Which part of the microscope will be used first to adjust the focus when starting with the lowest-power lens?

Coarse focus

Staining cells such that their color is different from the background enhances which quality of the cells?

Contrast

What is enhanced when a stain is used to make a sample, or parts of a sample, darker than the background in a slide?

Contrast

Which of the following is defined as the ability to distinguish an object from the background?

Contrast

Resolution is an image quality factor that involves being able to see fine ________, which is essential for image clarity.

Details

Which of these challenges are microscopes used to overcome?

Many biological specimens are too small to see with the unaided eye.

Which metric unit is most appropriate for expressing the size of human skin cells?

Micrometer

The total magnification of an image is determined by ______ the magnification power of the ocular lens and the magnification power of the objective lens used.

Multiplying

The lens on the microscope that is primarily responsible for the magnification of the specimen is the ____ lens.

Objective

The goal of microscopy is to create a magnified image of

Objects that are too small to be seen with the unaided eye.

Which part of the bright-field microscope is a lens that is located closest to the eye during use?

The ocular lenses

What is the name for the part of a bright-field microscope that displays the specimen?

The stage

What is the goal of microscopy?

To create a magnified image of objects that are too small to be seen with the unaided eye

The portion of a slide that is visible through the eyepiece is called the field of

View

The portion of a slide that is visible through the eyepiece is called the field of:

View

Resolution is an image quality factor that involves being able to see fine _______ , which is essential for image clarity.

Wet mount

Which microscopy method is beneficial because of its applications for living specimens?

Wet mount

The function of immersion oil in microscopy is to

direct more light rays to the objective lens.

Bright-field microscopes use a combination of lenses and ________ to view a specimen.

lights

Select the components used by a bright-field microscope to view a specimen.

1. A light source 2. Glass lenses

Select the specimen that could be viewed using a light microscope.

1. Cells 2. Tissues 3. Bacteria

Which metric units are most appropriate for expressing the size of macroscopic organisms?

1. Meters 2. Centimeters

Select the benefits of wet mount sample preparation for microscopy.

1. Motility of specimens can be observed. 2. The preparation is relatively simple and fast. 3. Living organisms can be viewed.

4x 10x 40x 100x

1. Scanning objective 2. Low power objective 3. High power objective 4. Oil immersion

Select all of the following that can be studied with bright-field microscopy.

1.Bacteria 2. Tissues 3. Cells

If a specimen is viewed using a 100× objective and the ocular lenses, what is the total magnification?

1000x

Oil immersion microscopy uses which objective lens?

100x lense

Which objective lens is used with a sample that has been prepared with a wet mount?

10× low-power lens

A benefit of using the oil immersion lens is a reduction in scatter, which

Field of view

The portion of a slide that is visible through the eyepiece is called the

Field of view

Bright-field microscopes use a combination of

Glass lenses and light to view a specimen.

Which lens has a magnification of 40×?

High-power objective

Changing the magnification of an image with a microscope changes the portion of the slide that is visible, or the

Increase resolution

Which phenomenon is reduced by oil immersion microscopy?

Loss of light that scatters into the air or onto the slide

Which lens has a magnification of 10×?

Low power objective

If you are unable to see something with your eyes, you can increase the _____ to make it look bigger.

Magnification

Which lens has a magnification of 100×?

Oil immersion

Increasing the _____ would allow you to tell if what appears as one object is really two objects extremely near each other.

Resolution

Which image quality factor is defined as the ability to distinguish fine detail?

Resolution

Which part of the bright-field microscope focuses light on the specimen?

The condenser