Microbiology Chapter 2

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brightfield microscope

A microscope that uses multiple lenses is called a _______ microscope.

fluorochromes

Chromophores that absorb and then emit light are called ________.

atomic force microscope

In a(n) __________ microscope, a probe located just above the specimen moves up and down in response to forces between the atoms and the tip of the probe?

gram staining

The ______ is used to differentiate bacteria cells based on the components of their cell walls.

tem is thin sem can be thicker

What is one difference between specimen preparation for a transmission electron microscope (TEM) and preparation for a scanning electron microscope (SEM)?

400x

What is the total magnification of a specimen that is being viewed with a standard ocular lens and a 40x objective lens?

iodine

What mordant is used in gram staining?

a brightfield microscope

What type of microscope uses a cone of light so that light only hits the specimen indirectly, producing a darker image on a brighter background?

a phase-contrast microscope

What type of microscope uses an annular stop?

refraction

When you see light bend as it moves from air into water, you are observing _______.

light with a short wavelength

Which of the following has the highest enegery?

a confocal scanning laser microscope

Which type of microscope is especially useful for viewing thick structures such as biofilms?

a scanning electron microscope

Which type of microscope would be the best choice for viewing very small surface structures of a cell?

a darkfield microscope

Which would be the best choice for viewing internal structures of a living protist such as a Paramecium?

Galileo Galilei

Who is the probable inventor of the compound microscope?

Robert Hooke

Who was the first to describe "cells" in dead cork tissue?

flourescent

You place a specimen under the microscope and notice that parts of the specimen begin to emit light immediately. These materials can be described as __________.

acid fast

Ziehl-Neelsen staining, a type of ________ staining, is diagnostic for Mycobacterium tuberculosis.


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