Aseptic Technique

Why was one sterile broth inoculated with E. coli and the other was not?

The tube that did not have bacteria added serves as a negative control to ensure that the broth and test tubes are sterile

Place the following steps of the aseptic transfer technique into the correct order

1. Pick up and heat loop 2. Pick up culture, remove cap, heat mouth of tube 3. Insert loop into culture 4. Heat mouth of culture tube, replace cap, place in rack 5. Lift lid and inoculate a sterile agar plate 6. Heat loop and return to rack

Place the following steps of the aseptic transfer technique for agar slant into the correct order

1. Pick up and heat loop 2. Pick up culture, remove cap, heat mouth of tube 3. Insert loop into culture 4. Heat mouth of culture tube, replace cap, place in rack 5. Pick up sterile slant, remove cap, heat mouth of tube 6. Streak culture onto sterile slant with loop 7. Heat mouth of sterile slant, replace cap, place in rack 8. Heat loop and return to rack

Pure culture

A bacterial culture containing only one strain of microorganism

Liquid broth culture

A liquid nutrient medium used for the propagation of microorganisms

Aseptic technique

A set of procedures employed in the handling of microbes without causing contamination from outside sources

Petri dish

A shallow, transparent glass or plastic dish that is covered by a larger lid

Inoculum

A small amount of microorganism used to start a new culture

Inoculating loop

A sterilizable tool made of wire, used to implant or move microorganisms

Colony

A visible mass of microorganisms formed by replication of a single microorganism on an agar surface, often in a petri dish

Sterile

An environment devoid of any living cells

Agar slant

Bacterial culture medium containing agar in a test tube that is allowed to solidify at an angle and forms a solid, slanted surface

Agar plate

Bacterial culture medium containing agar poured into a petri dish

Agar media

Bacterial culture medium that contains agar as a solidifying agent and is used for growing microorganisms

Which of the following are correct ways to label the organism found in a culture?

E. coli Escherichia coli

Which of the following best describes the result you see on the plate?

It shows a successful transfer of E. coli from a broth onto an agar plate

Where is the label placed on a petri plate?

On the bottom of the plate

Which of the following patterns is used to streak an agar slant?

Pass the loop across the surface of the slant