Mice in Research
What is the life span of a mouse? Max?
1-3 years; 4 years
What are the different Genetically Modified Mice?
- Hybrid mice - Trangenic mice - Knockout mice - Knockin mice Immunodefienct mice
Why is the mouse the most common lab animal used?
- Inexpensive - Readily available - Efficient breeders - Genome easily manipulated - Variety of research applications (genetics, cancer, infectious diseases studies)
Why can't mice and other rodent vomit?
- They lack the neural pathways to vomit - the connection of the esophagus at the stomach prevents regurgitation of feed
How much water does a mouse consume?
1.5 mL/10 gm body weight
How much food does a mouse consume?
12 gm/100 gm body weight
What is the normal respiration rate of a mouse
163 breaths per minute
What is normal weight of a Male mouse? Female?
20-40 gm 18-35 gm
What is the *Dental Formula* of a mouse?
2X (I 1/1, C 0/0, PM 0/0, M 3/3)
What is the heart rate of a mouse?
310-840 bpm
How many chromosomes do mice have?
40
What is a *Hybrid mice*?
Animals from 2 different strains are bred together to produce an animal with a mixture of genes from each strain
What are the most common strains of Inbred mice?
C57BL/6, BALB/c, C3H, FVB, 129 and DBA
Who developed the first Inbred mouse strain?
Clarence Little (From william Castle's lab)
What was the name of the first Inbred mice?
DBA mice - the birth of the first modern lab mouse 1909
What parts of the rodent body is responsible for their limited ability to dissipate heat?
Ears and Tails
What does the Nomenclature of *Inbred mice* look like?
Inbred mice referred to by a combination of letters and numbers EX: FVB, C3H, 129
Do mice and other rodents vomit?
No
Do mice have baby teeth?
No! only adult life
Do rodents possess sweat glands?
No, they also cannot pant
What are examples of *Immunodeficient mice*?
Nude, SCID, Rag-1
In an ____________________, not every mouse shares the same genes but the overall genetic makeup of groups remains stable from experiment to experiment
Outbred stock
What do mice do in response to warm temperatures?
Salivate
What does excessive secretion of the *Harderian gland* suggest?
Stress or illness
What are some examples of Outbred mouse stocks?
Swiss Webster, CD-1, and ICR
When do mice Incisors stop growing?
They don't, the grow throughout their life
How are *Hybrid mice* created?
When a first generation (F1) crosses between 2 different inbred strains
What is the *Harderian gland*?
a horseshoe shaped gland located within the orbit that produces secretion (reddish pigment porphyrin) that lubricates the globe.
What is a *Knockin mouse*?
a method in which a exogenous gene is introduced into the *mouse* genome to analyze product function and/or expression pattern
What is are *Knockout mice*?
a mouse whose DNA has been genetically engineered so that it does not express particular proteins
What does an *Outbred stock* produce?
a population with a "stabilized" genetic composition
How are *Inbred Strains* developed?
by breeding related individuals for many generation, until thee animals produced all have nearly the same genetic composition
How are *Outbred stocks* created?
by mating unrelated animals in a closed colony
How do mice respond to heat behaviorally?
by retreating into a burrow
Genetic similarity of Inbred mice strain have is like ___________?
identical twins
What are *Transgenic mice*?
mice that carry foreign DNA that was inserted into their own DNA
How do mice respond to cold?
non-shivering thermogenesis
When can Neonates control their body temp (ectpthermic)?
past 20 days
What does the Nomenclature of *Outbred mice* look like?
the outbred mice referred to by a laboratory code followed by a colon and stock designation EX: Crl:ICR
Why are *Transgenic mice* valuable in research?
they can be used to study human diseases that would not otherwise occur in unmodified mice
Due to the face that mice and other rodents can vomit, they can do what before anesthesia?
they can feed and water normally
