ASCP MLT Exam

VIII, IX, XI, XII, PK, HMWK

Intrinsic pathway

cystic fibrosis

Iontophoresis is a technique used in the diagnosis of

resistant

Jk(a-b-) red cells are __________ to lysis in 2 M urea

VP +

Klebsiella, Enterobacter, Serratia

HHMM

LD 3

Alder-Reilly

Large, darkly staining cytoplasmic granules composed of partially digested mucopolysaccharides

Spleen

Last resort treatment of ITP is removal of

Cold agglutinins

Least likely to interfere with hemoglobin measurements

Kappa or lambda

Light chains can be comprised of

Apoenzyme

Portion of enzyme separated from its cofactor

acetylsalicylic acid

Reye's syndrome

37C

Rh antibodies react best at

Diffuse cutaneous scleroderma

Scl-70+

Dohle bodies

Seen in severe infections and burns

Candida dubliniensis

Seen in thrush in aids patients

blue black

Starch stains ______________________ with iodine

hemoglobinopathy

Substitution of an amino acid in the hemoglobin chain

20-24C

The proper storage requirements for granulocyte concentrates is

budding

The stage of viral replication where the envelope is being acquired and the HIV is leaving the host cell is known as

1-2 minute break

The suggested time for a break after 30 minutes of computer use is

teardrop

These cells are associated with myelofibrosis

ALT

This test is used in the evaluation of hepatic disease

Francisella tularensis

Tiny, weak staining, gram negative coccobacilli

45%

Transferrin level that indicates to investigate further for hereditary hemochromatosis

Non-nucleated red blood cells

When performing a bone marrow analysis, which cell type is not included in the differential?

Liver

Where does the first hydroxylation of vitamin D occur?

Kidneys

Where does the second hydroxylation of vitamin D occur?

haptoglobin

Which blood level will decrease during intravascular hemolysis?

Arbitrary primed

Which derivative of PCR testing uses short primers not specifically complementary to a particular sequence of a target DNA?

hepatitis

Which infectious agent represents the greates risk to the laboratory worker?

transcription-mediated amplification

Which modified PCR technique or other method of amplification is used to detect Mycobacterium tuberculosis?

FISH

Which molecular method uses fluorescent probes to evaluate genes and/or DNA sequences on chromosomes?

aldolase

Which of the following enzymes is associated with conditions affecting skeletal muscles?

pancreatitis

Which of the following is considered a cause of hypomagnesemia?

Nasopharyngeal swab

Which of the following is the most suitable specimen for the isolation of Bordetella pertussis?

fructosamine

Which of the following methods for long-term diabetic monitoring is recommended for patients with sickle cell anemia?

proteins

Which of the following types od molecules is MOST LIKELY to be antigenic/immunogenic? nucleic acids carbohydrates lipids proteins

malaria

Which of the following viruses or diseases is screened for in blood donors only using questions asked during the donor screening and selection process?

TH2

Which subset of effector lymphs is predominately responsible for regulation of antibody production?

Immunoelectrophoresis

Which test confirms the presence of Bence-Jones proteinuria?

Troponin T

Which troponin protein binds the troponin complex to tropomyosin?

CEA

Which tumor marker is used for colorectal cancer?

CK-MB and myoglobin

Which two of the following biomarkers are not specific to cardiac muscle and may be elevated in patients with injury to muscle other than cardiac muscle?

Anti-A,B

Which type of antibody can cause HDFN in any pregnancy (first or subsequent), but is usually limited to less severe symptoms?

LDL

Which type of lipoprotein transports the MAJORITY of cholesterol to cells for steroid hormone synthesis or cell membrane incorporation?

Ionized calcium

Would be decreased due to delay in centrifugation

30 ml whole blood

a full 300 ug dose on RhIg provides protection for

8 weeks

accepted interval between whole blood donations

CIN

agar selective for Yersinia entercolitica

EBV virus

asymptomatic, >20% reactive lymphs

aminoglycosides

-mycins

Calcium

8-Hydroxyquinoline and CPC reagent is commonly used in the determination of

Color Doppler

BEST correlates with the severity of HDFN

1:10

Best dilution for slightly hazy CSF

dark field microscopy

Best suited for direct examination of infectious agent of syphilis

shaggy star

Candida parapsilosis

rosette clusters

Cardiobacterium hominis

indole pos

Chryseobacterium spp.

Treponemes

Direct examination can be performed by darkfield microscopy examination

Diphyllobothrium latum

Double genital pores emanating from each of the proglottids

VII, III

Extrinsic pathway

Leucine

Found in maple syrup urine disease

Necator americanus

Hookworm, long buccal cavity in rhabditiform

room temperature

How should a PT be stored until it is picked up by a courier?

Scopulariopsis

Hyaline mold that produces conidia in chains

Magnesium

Important metallic activator of alkaline phosphatase and creatine kinase

HbA

In the heterozygous state of HbS, sickle cell trait, which of the following hemoglobins appear in combination with HbS?

Obstructive jaundice

Increased alkaline phosphatase is found in

LDL-C

Increased level of ___________ indicates an increased risk of cardiovascular disease

Pneumocystis jiroveci

Increased risk of contracting this unusual form of pneumonia in AIDS patients

thrombosis

Obstruction of a blood vessel caused by a stationary blood clot is called

Acute glomerulonephritis

Oliguria is usually correlated with

Leb

Only this antigen adsorbs onto the red cells of adults

mariner's wheel

Paracoccidioides brasiliensis

carcinoid tumor

A markedly elevated 5-HIAA determination is presumptive evidence for

4 weeks

A potential donor who has received a German measles (rubella) vaccine is deferred for

HDN

A spectrophotometric scan of amniotic fluid may be valuable in the determination of which of the following conditions?

urine, serum, and whole blood

Acetest can be used to test for ketones in

HbS polymerization

Acid ph, dehydration, increased level of 2,3-DPG increases risk of

Lavender

Acinetobacter baumannii are ________________ on MAC agar

18-30 hours

After an AMI myoglobin levels will rise within 3 hours and peak at 8-12 hours. When will they return to normal?

Mild proteinuria

All of the following are urinalysis findings associated with nephrotic syndrome except: Oval fat bodies Fatty and waxy casts Renal tubular epithelial cells Mild proteinuria

I, II, V, X, XIII

All pathways

acute hemolytic transfusion reactions

Antibodies directed against granulocyte antigens have been implicated for all of the following except

IgG

Antibody class of paroxysmal cold hemoglobinuria

Haptoglobin

Appears in the alpha-2-globulin band

5-7

Approximately how many doses are required to obtain a steady state oscillation allowing for peak and trough levels of be evaluated?

Flavus

Aspergillus species that is granular to wooly with some shade of yellow or yellow-green

Thromboxane A2

Aspirin ingestion prevents the synthesis of this signaling molecule in the platelet

Burkholderia cepacia

Motile with polar tufts of flagella and can produce yellow pigment

CD2, 11b, 16

NK cells express

Anti-HBc

Necessary test to detect patients in the window period of a hepatitis B infection

Flavobacterium

Needs xylene extraction and Ehrlich's reagent for indole test

sucrose

Not considered a reducing sugar

Lewis

Not expressed, or weakly expressed on cord blood cells

lower, higher

Potassium, calcium, and total protein are ___________ in capillary specimens, where glucose is typically _______________ than in venous blood.

Kidd

Proteolytic enzyme techniques may be useful in identifying which of the following antigen groups?

zero order

Reaction depends only on enzyme concentration

polychromatic normoblast

Red cells begin forming enough hemoglobin to be visualizes on a wright stained bone marrow aspirate smear

primary myelofibrosis

The JAK2 mutation is seen in

flow cytometry

The MOST reliable method for determining the appropriate dosage of Rh immune globulin to give to an identified Rh immune globulin candidate after delivery is

gp41 or gp160

The Western Blot for HIV-1 infection identification looks for HIV p24 and what other two protein bands to confirm HIV-1 infection?

HBeAg

The antigen marker most closely associated with transmission of HBV infections is

Health hazard

The blue section of fire diamond means

B lymphs

The causative agent of infectious mono attaches to a receptor on which of the following cells?

sporothrix schenckii

The dimorphic fungi that may produce black, yeast-like colonies after prolonged incubation at 37C is

acetoacetic acid

The ketone component that is measured by the nitroprusside reaction

Pentatrichomonas hominis

Trophozoite is the only morphologic form in the life cycle of

Mixed connective tissue disease

U1RNP+

meat diet

Urine test strip positive for blood may indicate all except

Rabbit erythrocyte stroma

What absorption technique removes cold (IgM) antibodies, particuarly anti-I specificities?

NAD+

What coenzyme does LD use when converting lactate to pyruvate?

coomassie brilliant blue 250

What dye-binding method uses an increase of absorbance at 595 nm to determine the total protein concentration of the specimen?

extravascular

What form of hemolysis is seen in hereditary spherocytosis?

CDe/ce

What genotype is found with the highest frequency in caucasian population?

18-23 days; it binds with neonatal Fc receptor

What is the half-life of IgG? Why is the duration of the half-life significant?

myocardial ischemia

What is the term used to describe inadequate blood supply that decreases availability of oxygen to the myocardium?

1.5-2 mmol/L

What is the toxic level of lithium?

interferon-gamma release assays

What method is used to identify prior exposure to Mycobacterium tuberculosis?

platelet neutralization test

What test should be performed to determine if the patient has lupus anticoagulant?

monitoring course of treatment

benefits of RPR or VDRL tests

glucuronic acid

conjugates bilirubin

Sporothrix

daisy-like clusters produced from the tips of straight, delicate conidiosphores

aspirin

does not cause abnormal TT

reptilase

evaluate the adequacy of fibrinogen in heparinized patients

absence

high specificity means to accurately identify the _________________ of disease

brown/black urine

homogentisic acid

Hepatocellular carcinoma

increased AFP in adults

fibrinogen

most responsible for increasing the sed rate

CSF3R

mutation in chronic neutrophilic leukemia

exserohilum

nipple-like protrusion

speckled

no staining in metaphase mitotic cells

Rickettsia

obligate intracellular parasite

0.04 to 0.08

pH alteration of normal blood in a closed vessel standing at 37C for 1 hour is approximately

Bordatella pertussis

potato-blood-glycerol agar

LD50

the dose of drug in which 50% of the individuals will result in mortality

adenine and guanine

uric acid is the final breakdown product of what two purines?

Pulsed field electrophoresis

used to separate larger DNA fragments

diabetes

uses microalbuminuria to monitor