Exam 3 Study Questions (Part 2)
Dynein does not interact directly with a membrane bound cargo, but requires an intervening protein complex called:
Dynactin
This GTP-binding protein is used to release a clathrin-coated vesicle from the membrane from which it was formed:
Dynamin
The motor protein that goes from the plus to the minus end of microtubules is:
Dynein
If a protein passes through a TOM complex, we can conclude that this protein was translated:
In the cytosol
The word used to specifically indicate the uptake of large particles (as opposed to taking up just liquid or internalizing cell surface receptors) is:
Phagocytosis
Proteins in the RER are subject to quality control; if they are not folded correctly after several attempts, they are removed from the RER by reverse translocation and destroyed by:
Proteasomes
What is NOT true of Kinesin?
-It travels to the minus end of microfilaments. -It travels to the plus end of microfilaments. -It falls off microtubules easily. -It is not highly processive.
Which events are NOT involved in the unfolded protein response?
-Less new chaperones are made -General translation is increased
Which proteins are synthesized using free cytosolic ribosomes (as opposed to on the RER)?
-Nuclear -Mitochondrial
Microtubules are made up of:
A tube of 13 protofilaments Tubulin dimers
What protein is the most important in phagocytosis?
Actin
Clathrin forms a honeycomb lattice in order to form vesicles and are connected to the membrane through protein complexes called:
Adaptors
N-linked oligosaccharides are bonded to this amino acid:
Asparagine
Organelle turnover is accomplished through:
Autophagy
In protein synthesis, a stop-transfer sequence indicates that a protein will:
Become an integral protein
Molecules are moved from the RER to the Golgi via transport vesicles. The protein that mediates the budding on the RER to make the transport vesicle is called:
COPII
In animals, the microtubules of the cytoskeleton are nucleated by a complex structure that contains centrioles surrounded by PCM. These nucleating structures are called :
Centrosomes
What model is favored to describe the dynamics of transport through the Golgi complex?
Cisternal maturation model
In endocytosis a vesicle is formed from the plasma membrane using these triskelion protein complexes called:
Clathrin
Which protein coats the vesicles that carry mannose 6-phosphate receptors from the Golgi?
Clathrin
A signal recognition particle is NOT capable of:
Cleaving a signal peptide
COPI, COPII and Clathrin are proteins that can be found on:
Coated vesicles
Where are proteasomes found?
Cytosol
Formation of N-linked oligosaccharides begins in the RER, not on the protein itself, but rather on a lipid called __________ phosphate, which later donates its oligosaccharide to the protein.
Dolichol
Dynamic instability refers to the assembly/disassembly properties of the plus end of a microtubule, which can switch back and forth between growing and shortening phases. The assembly of tubulin dimers onto the growing microtubule requires the presence of:
GTP
G proteins are:
GTP-binding proteins
A particular protein component specific to all MTOCs is:
Gamma tubulin
What protein is synthesized on the rough endoplasmic reticulum (RER)?
Integral (Proteins for export out of the cell)
Autophagy is a process where an organelle:
Is surrounded by a membrane from the ER and then fuses with a lysosome
Name a common skin protein that forms intermediate filaments:
Keratin
If a cell has its MTOC located near the cis-Golgi, what motor would most likely be used to transport clathrin coated vesicles away from the trans-Golgi?
Kinesin
The KDEL retrieval signal consists of (in order):
Lysine, aspartic acid, glutamic acid and leucine
The KDEL retrieval signal consists of:
Lysine, aspartic acid, glutamic acid and leucine
Acid hydrolases are characteristic of which organelle?
Lysosome
Organelles with a low pH and possessing acid hydrolases that are used to digest macromolecules are called:
Lysosomes
Besides alpha and beta tubulin, microtubules may contain what proteins for cross-linking and stabilization?
MAPs
Any structure (example: centrosome) that is used by the cell to initiate microtubule formation is called an:
MTOC
In what way does rough endoplasmic reticulum (RER) differ from smooth endoplasmic reticulum (SER)?
RER has ribosomes: SER does not.
COPII-coated vesicles are involved in moving materials from the:
RER to the ERGIC
The concept that proteins possess amino acids that act as an address code is called the:
Signal hypothesis
What complex allows proteins to move from the cytosol through the inner mitochondrial membrane?
TIM
What complex allows proteins to move from the cytosol through the outer mitochondrial membrane?
TOM
What statement correctly explains how cilia move?
The dynein arms on an A tubule pulls against the B tubule of a neighboring doublet.
If a protein synthesized by a free ribosome has a targeting sequence for the lumen of the chloroplast, what membrane protein complex will participate in its importation?
Tic
The membrane that envelopes the plant vacuole is called a:
Tonoplast
What "ball" shows the best model for how clathrin forms its shape?
A soccer ball
Coated pits are coated on their cytoplasmic side by:
Clathrin
O-linked oligosaccharides are bonded to what amino acid:
Threonine
What network is closest in proximity to the RER?
cis Golgi network