Exam 3 Study Questions (Part 2)

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Dynein does not interact directly with a membrane bound cargo, but requires an intervening protein complex called:

Dynactin

This GTP-binding protein is used to release a clathrin-coated vesicle from the membrane from which it was formed:

Dynamin

The motor protein that goes from the plus to the minus end of microtubules is:

Dynein

If a protein passes through a TOM complex, we can conclude that this protein was translated:

In the cytosol

The word used to specifically indicate the uptake of large particles (as opposed to taking up just liquid or internalizing cell surface receptors) is:

Phagocytosis

Proteins in the RER are subject to quality control; if they are not folded correctly after several attempts, they are removed from the RER by reverse translocation and destroyed by:

Proteasomes

What is NOT true of Kinesin?

-It travels to the minus end of microfilaments. -It travels to the plus end of microfilaments. -It falls off microtubules easily. -It is not highly processive.

Which events are NOT involved in the unfolded protein response?

-Less new chaperones are made -General translation is increased

Which proteins are synthesized using free cytosolic ribosomes (as opposed to on the RER)?

-Nuclear -Mitochondrial

Microtubules are made up of:

A tube of 13 protofilaments Tubulin dimers

What protein is the most important in phagocytosis?

Actin

Clathrin forms a honeycomb lattice in order to form vesicles and are connected to the membrane through protein complexes called:

Adaptors

N-linked oligosaccharides are bonded to this amino acid:

Asparagine

Organelle turnover is accomplished through:

Autophagy

In protein synthesis, a stop-transfer sequence indicates that a protein will:

Become an integral protein

Molecules are moved from the RER to the Golgi via transport vesicles. The protein that mediates the budding on the RER to make the transport vesicle is called:

COPII

In animals, the microtubules of the cytoskeleton are nucleated by a complex structure that contains centrioles surrounded by PCM. These nucleating structures are called :

Centrosomes

What model is favored to describe the dynamics of transport through the Golgi complex?

Cisternal maturation model

In endocytosis a vesicle is formed from the plasma membrane using these triskelion protein complexes called:

Clathrin

Which protein coats the vesicles that carry mannose 6-phosphate receptors from the Golgi?

Clathrin

A signal recognition particle is NOT capable of:

Cleaving a signal peptide

COPI, COPII and Clathrin are proteins that can be found on:

Coated vesicles

Where are proteasomes found?

Cytosol

Formation of N-linked oligosaccharides begins in the RER, not on the protein itself, but rather on a lipid called __________ phosphate, which later donates its oligosaccharide to the protein.

Dolichol

Dynamic instability refers to the assembly/disassembly properties of the plus end of a microtubule, which can switch back and forth between growing and shortening phases. The assembly of tubulin dimers onto the growing microtubule requires the presence of:

GTP

G proteins are:

GTP-binding proteins

A particular protein component specific to all MTOCs is:

Gamma tubulin

What protein is synthesized on the rough endoplasmic reticulum (RER)?

Integral (Proteins for export out of the cell)

Autophagy is a process where an organelle:

Is surrounded by a membrane from the ER and then fuses with a lysosome

Name a common skin protein that forms intermediate filaments:

Keratin

If a cell has its MTOC located near the cis-Golgi, what motor would most likely be used to transport clathrin coated vesicles away from the trans-Golgi?

Kinesin

The KDEL retrieval signal consists of (in order):

Lysine, aspartic acid, glutamic acid and leucine

The KDEL retrieval signal consists of:

Lysine, aspartic acid, glutamic acid and leucine

Acid hydrolases are characteristic of which organelle?

Lysosome

Organelles with a low pH and possessing acid hydrolases that are used to digest macromolecules are called:

Lysosomes

Besides alpha and beta tubulin, microtubules may contain what proteins for cross-linking and stabilization?

MAPs

Any structure (example: centrosome) that is used by the cell to initiate microtubule formation is called an:

MTOC

In what way does rough endoplasmic reticulum (RER) differ from smooth endoplasmic reticulum (SER)?

RER has ribosomes: SER does not.

COPII-coated vesicles are involved in moving materials from the:

RER to the ERGIC

The concept that proteins possess amino acids that act as an address code is called the:

Signal hypothesis

What complex allows proteins to move from the cytosol through the inner mitochondrial membrane?

TIM

What complex allows proteins to move from the cytosol through the outer mitochondrial membrane?

TOM

What statement correctly explains how cilia move?

The dynein arms on an A tubule pulls against the B tubule of a neighboring doublet.

If a protein synthesized by a free ribosome has a targeting sequence for the lumen of the chloroplast, what membrane protein complex will participate in its importation?

Tic

The membrane that envelopes the plant vacuole is called a:

Tonoplast

What "ball" shows the best model for how clathrin forms its shape?

A soccer ball

Coated pits are coated on their cytoplasmic side by:

Clathrin

O-linked oligosaccharides are bonded to what amino acid:

Threonine

What network is closest in proximity to the RER?

cis Golgi network


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