Microscopy- Use and Function in Anatomy
Scanning electron microscope
A _____ produces images by reflecting electrons from the surface of the sample back to the detector.
A dissecting miscroscope
A stereoscopic microscope is also known as ----
An optical microscope
A transmission electron microscope is similar in design to a(n)----
Stereoscopic microscope
A(n) _____ allows an entire specimen to be viewed in 3D by reflecting light off of the specimen.
electrons
An electron microscope uses --- to illuminate an object and create an image
Yes
Is a compound microscope a type of optical microscope?
No
Is the depth of view independent of the total magnification factor?
Diphragm
The --- sits below the stage and varies the field for viewing the specimen
Objective lens
The _____ lenses are the scanning power, the low power, and the high power.
Scanning probe microscope
The _____ traces the surface of the sample in the microscope with a small probe.
Greater
The larger the apex of the probe, the ----- the resolution of the scanning probe microscope
fine adjustment knob
The main component of an optical microscope that is used to focus an image at a high power of magnification is the
Stage
The slide is placed on the _____ of an optical microscope.
Compound microscope
The_____ utilizes a lens of short focal length and a second lens of a longer focal length to magnify and focus an object.
True
True or False: Depth of field refers to the distance range that is in focus at a given time.
False
True or False: The scanning power is the highest power of magnification of the compound microscope.
False
True or False: When creating a wet-mount slide, an individual should drop the cover slip on the top of a water droplet placed on the slide without touching it to the slide first.
Also called light microscopes, use reflected visible light and a series of two or more convex lenses to magnify and focus an object.
What are optical microscopes?
Optical, electron, and scanning probe
What are the three main categories of microscopes?
movable platform that allows the user to move the slide left and right, or forward and backward, by turning the stage adjustment knobs
What is a mechanical stage?
An optical instrument that uses a lens or a series of lenses to magnify objects that are either too small to be seen by the naked eye, or to view distinct details of an object that are not visible to the naked eye.
What is a microscope?
Does not transmit the beam of electrons through the sample; rather it produces images by reflecting electrons from the surface of the sample back to the detector. Produces 3D objects in black and white
What is a scanning electron microscope?
Uses a very small, very sharp probe (3 - 50 nm in curvature) to trace the surface of the sample in the microscope
What is a scanning probe microscope?
Uses a lower power of magnification, allowing an entire specimen, to be viewed in 3D by reflecting light off of the specimen. Also called a dissecting microscope
What is a stereoscopic microscope?
Transmits the beam of electrons through the very thin sample. Transmission electron microscopes produce only black and white images and only allow for two-dimensional views of the sample
What is a transmission electron microscope?
A revolving nose peice
What is a turret?
the distance range that is in focus at a given time
What is depth of field?
Will include most of the foreground and background
What is greater focus?
the ability to discern two objects as separate, and is limited by the wavelength of light.
What is resolving power?
Emphasizes a subject
What is shallow focus?
Tip of the probe that dictates the resolution of the scanning probe microscope
What is the apex of a scanning probe microscope?
supports the lenses of the microscope and connects them to the base of the microscope
What is the arm of microscope?
bottom of the microscope, which provides support to the instrument
What is the base?
focuses light on specimen
What is the condenser lens?
used when larger amounts of focus are needed and moves the stage a greater distance
What is the course adjustment knob?
sits below the stage and the condenser lens. It is composed of overlapping pieces of metal or plastic that move to vary the amount of light projected toward the condenser lens.
What is the diaphragm?
used for focusing at higher powers of magnification and moves the stage in very small increments
What is the fine adjustment knob?
highest strength objective lens (40x)
What is the high power lens?
Light source which illuminates the object and shines into the lens
What is the illuminator?
Medium strength objective lens (10x)
What is the low power lens?
Compound light microscope
What is the most common microscope?
Lens closest to the eye
What is the ocular lens?
Fourth objective lens (100x) if present
What is the oil immersion lens?
lowest power objective lens (4x)
What is the scanning lens?
Holds the microscope slide in position.
What is the stage?
The magnification of the ocular lens multiplied by the magnification of the objective lens
What is the total magnification factor?
