Lab Practicum #2 Question Set - 3. Urease Test (Urea Hydrolysis)

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Right tube

Which tube is positive in the Urease test?

Proteus mirabilis and Proteus vulgaris cause diaper rash

26. What other disease is caused by this organism? (refers to #25)

ammonia (NH3) urease

24. Urea can be hydrolyzed to carbon dioxide and ___________________ by bacteria that produce the enzyme ______________________________.

Proteus

25. Urinary tract pathogens from the genus ______________________ can be distinguished from other enteric bacteria by their rapid urease activity

ammonia, raises, orange/yellow to pink

27. Urease-positive organisms break down urea to _______________, which ________________ the pH of the medium and changes the color of the pH indicator phenol red from _______________ to _______________.

Positive - pink tube; bacteria uses urease to breakdown urea Negative - yellow/pale orange; no urease present

28. Describe a positive urease test and a negative test (include the relative pH).

increase buffer

29. In order for a urease-positive organism to turn the urea broth pink, the organism must ___________________ the pH of the medium enough to overcome the effects of the __________________, potassium phosphate.

Helicobacter pylori

30. The urease test is an important part of the identification of the organism_________________________ , that causes peptic ulcers.

secretes enzyme urease that neutralizes stomach acid so organisms can eat the muccous membrane

31. How does this organism (refers to #30) survive stomach acid?*

majority

32. A rapid urease test is performed on the ______________________ of patients with peptic ulcer symptoms.

to not obtain a false positive from reversion of peptone metabolism

33. Why is it important that urease broth contain a buffer that can resist alkalinization of the medium from peptone metabolism?*

nitrogen

34. Ammonia is used by many bacteria as a source of the element ______________________.

nucleic acids and protein

35. Bacteria use the element listed in #34, in order to make what two classes of macromolecules?*


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